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KMID : 0352720160400030220
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Journal of Ginseng Research
2016 Volume.40 No. 3 p.220 ~ p.228
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Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography-electron spray ionization-MS/MS
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Ting-Ting Sun
Xin-Lei Liang
He-Yun Zhu
Xu-Ling Peng
Xing-Jie Guo
Long-Shan Zhao
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Abstract
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Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng.
Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLCeMS/MS) method was developed to separate and identify saponins in Shizhu ginseng.
Results: The separation was carried out on a Waters ACQUITY UPLC BEH C18 column (100 mm 2.1 mm, 1.7 mm) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at 40C. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances.
Conclusion: The developed UHPLCeMS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng.
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KEYWORD
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identification, saponins, separation, Shizhu ginseng, ultra-high performance liquid chromatography coupled with MS/MS
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